If you want to find out about AAV packaging services, you are on the right page. These companies offer AAV services that include Vitro work, small-scale packaging, and large-scale packaging. Aside from this, this type of service also includes double AAV GMP especially for clinical trials. Regardless of your research, you will work with a team of scientists to provide AAV services to meet your needs. Let’s find out more.
AAV Packaging Services
You can choose from a lot of packaging services. The companies will be more than happy ye to customize the order based on your needs. However, it is important to keep in mind that the prices of the services and the delivery time will be different on the basis of the order complexity.
Why Go for AAV?
Although AAV is common in humans, know that it is quite non-pathogenic and has a mild immune response. Also, the wild type can get into the genome of the host. On the other hand, recombinant AAV does not have two vital genes as far as replication and viral integration is concerned.
So, the Recombinant AAV stays episomal and tends to persist in different non-dividing cells for a longer period of time. Based on these features and the ability to affect different types of tissues, the Recombinant AV is known as one of the primary viral vectors that are used for or conducting research.
Now let’s take a look at some advantages and disadvantages of AAV packaging services. In short, we can say that the pros of the service outweigh the cons. So, it’s a great deal.
Advantages
- Long Term Gene Expression
- Non Integrating
- Tissue-Based Targeting
- Low Immunogenicity
Disadvantages
- Not a Good Choice to make stable cell lines
- Lower Packaging Capacity
- Moderate Expression as far as Vitro is Concerned
- It’s important to keep in mind this is not the complete list of advantages offered by the service.
Recombinant AAV Packaging
It is important to know that they are associated with the Dependovirus genus. Therefore, it relies upon a helper virus to spread the infection. In addition, the Recombinant genome does not have two primary genes. Therefore AAV is an effective tool as far as gene delivery is concerned. For generating AAV particles, there is a need for essential genes.
Many service providers make use of the triple transfection strategy in order to provide AAV packaging. In this process, they co-transfect the desired packaging cell line with the given AAV plasmid that contains the GOI, rep genes, cap genes, and other stuff required for replication.
Most service providers purify AAV particles with gradient ultracentrifugation for the removal of empty capsids and impurities. Then the viral titer is determined with the help of qPCR and quantification on the basis of the ITRs contained in the viral genome. Afterward, the AAV particles are sent through an overnight shipment service for use in Vivo or Vitro research.
So, this was a basic introduction to AAV packing services and the important things you may want to consider. Hopefully, you will find this article useful.
